Appearance: light yellow solid Formula: C9H16O6PCl Molecular Weight: 286.65 Storage conditions: sealed and dark storage Melting point: 177 ± 2 ℃ Uses: protein disulfide reductant Characteristics
Tasteless - Contributing a healthy laboratory environment
Specificity - Targeted and completely reduce even the most stable disulfide solution
Simple – Effect at room temperature and PH value 5 within five minutes
Stability – Stability in air, It is not reaction with the functional group in protein, it is not volatile.
Application
Protein disulfide bond reducing agent, it also has an antioxidant properties. One of the main purposes of the TCEP is reducing anent or deprotecting agent for thiolated DNA. It is often use to make reduction of disulfide bonds in protein, It could be prevented a intra or inter molecular link of disulfide bonds between cysteine in proteins or between the proteins.
TCEP reducing ability is stronger then beta-mercaptoethanol and DTT, its effect maintained for 2-3 weeks. Both in the acidic or alkaline conditions, TCEP is more stable, better then DTT.
TCEP is tasteless, non-toxic, water-soluble, easier operation. Unlike DTT, It could not make the Ni ionic reduction, It is better compatibility with other biological agents and produce an easy condition for further mass spectrometric analysis.
Packing form:
1 kg plastic airtight drum, avoid the hot ,moisture and light. Or according to customer needs.
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